4R5Q

Crystal structure and nuclease activity of the CRISPR-associated Cas4 protein Pcal_0546 from Pyrobaculum calidifontis containing a [2Fe-2S] cluster

Replaces:  4ONB

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	CRISPR-associated exonuclease, Cas4 family	polymer	216	25031.3	1	UniProt (A3MTK6) Pfam (PF01930) Pfam (PF12705) In PDB	Pyrobaculum calidifontis JCM 11548
2	A	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
3	A	FE2/S2 (INORGANIC) CLUSTER	non-polymer		175.8	1	Chemie (FES)
4		water	water		18.0	21	Chemie (HOH)

Sequence modifications

A: 1 - 213 (UniProt: A3MTK6)

PDB	External Database	Details
Phe -2	-	EXPRESSION TAG
Asn -1	-	EXPRESSION TAG
Gly 0	-	EXPRESSION TAG

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	25031.3
Non-Polymers*	Number of molecules	2
	Total formula weight	200.1
All*	Total formula weight	25231.5

*Water molecules are not included.