4R5Q

Crystal structure and nuclease activity of the CRISPR-associated Cas4 protein Pcal_0546 from Pyrobaculum calidifontis containing a [2Fe-2S] cluster

Replaces:  4ONB

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000014	molecular_function	single-stranded DNA endodeoxyribonuclease activity
A	0004527	molecular_function	exonuclease activity
A	0006259	biological_process	DNA metabolic process
A	0032508	biological_process	DNA duplex unwinding
A	0045145	molecular_function	single-stranded DNA 5'-3' DNA exonuclease activity
A	0046872	molecular_function	metal ion binding
A	0051537	molecular_function	2 iron, 2 sulfur cluster binding
A	0051607	biological_process	defense response to virus

Functional Information from PDB Data

site_id	AC1
Number of Residues	5
Details	BINDING SITE FOR RESIDUE MG A 301

Chain	Residue
A	ASP123
A	GLU136
A	ILE137
A	HIS146
A	HOH407

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site_id	AC2
Number of Residues	5
Details	BINDING SITE FOR RESIDUE FES A 302

Chain	Residue
A	CYS209
A	CYS64
A	ALA198
A	CYS200
A	CYS203

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	4
Details	BINDING: BINDING => ECO:0000269|PubMed:25200083

Chain	Residue	Details
A	CYS64
A	CYS200
A	CYS203
A	CYS209

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site_id	SWS_FT_FI2
Number of Residues	4
Details	BINDING: BINDING => ECO:0000303|PubMed:25200083

Chain	Residue	Details
A	ILE137
A	HIS94
A	ASP123
A	GLU136

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