4QZI

Mouse Tdt, F401A mutant, in complex with a DSB substrate and Zn2+

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	DNA nucleotidylexotransferase	polymer	400	45627.9	1	UniProt (P09838) Pfam (PF14716) Pfam (PF10391) Pfam (PF01909) Pfam (PF14791)	Mus musculus (mouse)	Terminal addition enzyme, Terminal deoxynucleotidyltransferase, TDT, Terminal transferase
2	B, D (D, U)	5'-D(*AP*AP*AP*AP*AP*C)-3'	polymer	6	1810.3	2
3	C (T)	5'-D(*TP*TP*TP*TP*TP*GP*GP*G)-3'	polymer	8	2463.6	1
4	E, F (A)	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
5	G (A)	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
6	H (A)	SODIUM ION	non-polymer		23.0	1	Chemie (NA)
7	I (A)	2',3'-DIDEOXYCYTIDINE 5'-TRIPHOSPHATE	non-polymer		451.2	1	Chemie (DCT)
8	J, K, L, M (A, D, T, U)	water	water		18.0	70	Chemie (HOH)

Sequence modifications

A: 132 - 482 (UniProt: P09838)

PDB	External Database	Details
Met 111	-	expression tag
Gly 112	-	expression tag
Ser 113	-	expression tag
Ser 114	-	expression tag
His 115	-	expression tag
His 116	-	expression tag
His 117	-	expression tag
His 118	-	expression tag
His 119	-	expression tag
His 120	-	expression tag
Ser 121	-	expression tag
Ser 122	-	expression tag
Gly 123	-	expression tag
Leu 124	-	expression tag
Val 125	-	expression tag
Pro 126	-	expression tag
Arg 127	-	expression tag
Gly 128	-	expression tag
Ser 129	-	expression tag
His 130	-	expression tag
Met 131	-	expression tag
Ala 401	Phe 401	engineered mutation

A: 483 - 510 (UniProt: P09838)

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	51712.1
Non-Polymers*	Number of molecules	5
	Total formula weight	629.3
All*	Total formula weight	52341.3

*Water molecules are not included.