4QZC

Mouse Tdt, F405A mutant, in complex with a DSB substrate, C-G base pair

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	DNA nucleotidylexotransferase	polymer	400	45627.9	1	UniProt (P09838) Pfam (PF14716) Pfam (PF10391) Pfam (PF01909) Pfam (PF14791)	Mus musculus (mouse)	Terminal addition enzyme, Terminal deoxynucleotidyltransferase, TDT, Terminal transferase
2	B, D (D, U)	5'-D(*AP*AP*AP*AP*AP*C)-3'	polymer	6	1810.3	2
3	C (T)	5'-D(*TP*TP*TP*TP*TP*GP*G)-3'	polymer	7	2134.4	1
4	E, F (A)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
5	G (A)	SODIUM ION	non-polymer		23.0	1	Chemie (NA)
6	H (A)	2',3'-DIDEOXYCYTIDINE 5'-TRIPHOSPHATE	non-polymer		451.2	1	Chemie (DCT)
7	I, J, K, L (A, D, T, U)	water	water		18.0	126	Chemie (HOH)

Sequence modifications

A: 132 - 482 (UniProt: P09838)

PDB	External Database	Details
Met 111	-	expression tag
Gly 112	-	expression tag
Ser 113	-	expression tag
Ser 114	-	expression tag
His 115	-	expression tag
His 116	-	expression tag
His 117	-	expression tag
His 118	-	expression tag
His 119	-	expression tag
His 120	-	expression tag
Ser 121	-	expression tag
Ser 122	-	expression tag
Gly 123	-	expression tag
Leu 124	-	expression tag
Val 125	-	expression tag
Pro 126	-	expression tag
Arg 127	-	expression tag
Gly 128	-	expression tag
Ser 129	-	expression tag
His 130	-	expression tag
Met 131	-	expression tag
Ala 405	Phe 405	engineered mutation

A: 483 - 510 (UniProt: P09838)

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	51382.8
Non-Polymers*	Number of molecules	4
	Total formula weight	522.8
All*	Total formula weight	51905.6

*Water molecules are not included.