4QZC
Mouse Tdt, F405A mutant, in complex with a DSB substrate, C-G base pair
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-07-31 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 0.872600 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 56.397, 75.178, 125.943 |
Unit cell angles | 90.00, 99.05, 90.00 |
Refinement procedure
Resolution | 44.750 - 2.750 |
R-factor | 0.189 |
Rwork | 0.188 |
R-free | 0.21380 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4i2a |
RMSD bond length | 0.010 |
RMSD bond angle | 1.000 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.000 | 2.900 |
High resolution limit [Å] | 2.750 | 2.750 |
Rmerge | 0.069 | 0.639 |
Number of reflections | 13623 | |
<I/σ(I)> | 14.8 | 2.2 |
Completeness [%] | 99.9 | 100 |
Redundancy | 4.2 | 4.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 12-17% PEG4000, 9-12% isopropanol, 100 mM sodium acetate, 100 mM HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |