4QVH
Crystal structure of the essential Mycobacterium tuberculosis phosphopantetheinyl transferase PptT, solved as a fusion protein with maltose binding protein
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B | Maltose-binding periplasmic protein, 4'-phosphopantetheinyl transferase chimera | polymer | 598 | 65169.3 | 2 | UniProt (P0AEX9) UniProt (O33336) Pfam (PF01547) Pfam (PF17837) Pfam (PF01648) In PDB | Escherichia coli | MBP, MMBP, Maltodextrin-binding protein, Phosphopantetheinyl transferase PptT (CoA:APO-[ACP]pantetheinephosphotransferase) (CoA:APO-[acyl-carrier protein]pantetheinephosphotransferase) |
2 | C, D | alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose | branched | 342.3 | 2 | In PDB BIRD (PRD_900001) GlyTouCan (G07411ON) | alpha-maltose | ||
3 | A, B | COENZYME A | non-polymer | 767.5 | 2 | Chemie (COA) | |||
4 | A, B | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) | |||
5 | A, B | GLYCEROL | non-polymer | 92.1 | 7 | Chemie (GOL) | |||
6 | A, B | CITRATE ANION | non-polymer | 189.1 | 2 | Chemie (FLC) | |||
7 | water | water | 18.0 | 778 | Chemie (HOH) |
Sequence modifications
A, B: 2 - 367 (UniProt: P0AEX9)
A, B: 372 - 598 (UniProt: O33336)
PDB | External Database | Details |
---|---|---|
Met 1 | - | expression tag |
Ala 173 | Glu 198 | conflict |
Ala 174 | Asn 199 | conflict |
Ala 360 | Glu 385 | conflict |
Ala 363 | Lys 388 | conflict |
Ala 364 | Asp 389 | conflict |
Asn 368 | - | expression tag |
Ala 369 | - | expression tag |
Ala 370 | - | expression tag |
Ala 371 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 2 |
Total formula weight | 130338.5 | |
Branched | Number of molecules | 2 |
Total formula weight | 684.6 | |
Non-Polymers* | Number of molecules | 13 |
Total formula weight | 2606.5 | |
All* | Total formula weight | 133629.6 |