4QUM

Crystal structure of PTPN3 (PTPH1) in complex with a dually phosphorylated MAPK12 peptide

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Tyrosine-protein phosphatase non-receptor type 3	polymer	306	34887.7	1	UniProt (P26045) Pfam (PF00102) In PDB	Homo sapiens (human)	Protein-tyrosine phosphatase H1, PTP-H1
2	B	Mitogen-activated protein kinase 12	polymer	9	1244.3	1	UniProt (P53778) In PDB	Homo sapiens	MAP kinase 12, MAPK 12, Extracellular signal-regulated kinase 6, ERK-6, Mitogen-activated protein kinase p38 gamma, MAP kinase p38 gamma, Stress-activated protein kinase 3
3		water	water		18.0	27	Chemie (HOH)

Sequence modifications

A: 628 - 909 (UniProt: P26045)

PDB	External Database	Details
Met 604	-	expression tag
His 605	-	expression tag
His 606	-	expression tag
His 607	-	expression tag
His 608	-	expression tag
His 609	-	expression tag
His 610	-	expression tag
Ser 611	-	expression tag
Ser 612	-	expression tag
Gly 613	-	expression tag
Val 614	-	expression tag
Asp 615	-	expression tag
Leu 616	-	expression tag
Gly 617	-	expression tag
Thr 618	-	expression tag
Glu 619	-	expression tag
Asn 620	-	expression tag
Leu 621	-	expression tag
Tyr 622	-	expression tag
Phe 623	-	expression tag
Gln 624	-	expression tag
Ser 625	-	expression tag
Asn 626	-	expression tag
Ala 627	-	expression tag
Ala 811	Asp 811	engineered mutation
Ser 842	Cys 842	engineered mutation

B: 182 - 190 (UniProt: P53778)

PDB	External Database	Details
Arg 190	Trp 190	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	36131.9
All*	Total formula weight	36131.9

*Water molecules are not included.