4QPJ

2.7 Angstrom Structure of a Phosphotransferase in Complex with a Receiver Domain

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Phosphotransferase	polymer	243	25868.5	2	UniProt (Q2YQA5) Pfam (PF10090)	Brucella abortus
2	C, D (C, D)	Cell cycle response regulator CtrA	polymer	152	16871.4	2	UniProt (Q2YQA4) Pfam (PF00072)	Brucella abortus
3	E, F, I, J, M (A, B, C)	GLYCEROL	non-polymer		92.1	5	Chemie (GOL)
4	G (A)	SODIUM ION	non-polymer		23.0	1	Chemie (NA)
5	H, L, N (A, B, C)	CALCIUM ION	non-polymer		40.1	3	Chemie (CA)
6	K (B)	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
7	O (D)	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
8	P, Q, R, S (A, B, C, D)	water	water		18.0	23	Chemie (HOH)

Sequence modifications

A, B: 1 - 209 (UniProt: Q2YQA5)

PDB	External Database	Details
Met -33	-	initiating methionine
Gly -32	-	expression tag
Ser -31	-	expression tag
Ser -30	-	expression tag
His -29	-	expression tag
His -28	-	expression tag
His -27	-	expression tag
His -26	-	expression tag
His -25	-	expression tag
His -24	-	expression tag
Ser -23	-	expression tag
Ser -22	-	expression tag
Gly -21	-	expression tag
Leu -20	-	expression tag
Val -19	-	expression tag
Pro -18	-	expression tag
Arg -17	-	expression tag
Gly -16	-	expression tag
Ser -15	-	expression tag
His -14	-	expression tag
Met -13	-	expression tag
Ala -12	-	expression tag
Ser -11	-	expression tag
Met -10	-	expression tag
Thr -9	-	expression tag
Gly -8	-	expression tag
Gly -7	-	expression tag
Gln -6	-	expression tag
Gln -5	-	expression tag
Met -4	-	expression tag
Gly -3	-	expression tag
Arg -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Ile 28	Val 28	SEE REMARK 999

C, D: 1 - 118 (UniProt: Q2YQA4)

PDB	External Database	Details
Met -33	-	initiating methionine
Gly -32	-	expression tag
Ser -31	-	expression tag
Ser -30	-	expression tag
His -29	-	expression tag
His -28	-	expression tag
His -27	-	expression tag
His -26	-	expression tag
His -25	-	expression tag
His -24	-	expression tag
Ser -23	-	expression tag
Ser -22	-	expression tag
Gly -21	-	expression tag
Leu -20	-	expression tag
Val -19	-	expression tag
Pro -18	-	expression tag
Arg -17	-	expression tag
Gly -16	-	expression tag
Ser -15	-	expression tag
His -14	-	expression tag
Met -13	-	expression tag
Ala -12	-	expression tag
Ser -11	-	expression tag
Met -10	-	expression tag
Thr -9	-	expression tag
Gly -8	-	expression tag
Gly -7	-	expression tag
Gln -6	-	expression tag
Gln -5	-	expression tag
Met -4	-	expression tag
Gly -3	-	expression tag
Arg -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	85479.9
Non-Polymers*	Number of molecules	11
	Total formula weight	663.5
All*	Total formula weight	86143.3

*Water molecules are not included.