4QPJ

2.7 Angstrom Structure of a Phosphotransferase in Complex with a Receiver Domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2013-03-28
Detector	MARMOSAIC 225 mm CCD
Spacegroup name	P 32 2 1
Unit cell lengths	124.951, 124.951, 136.332
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	17.879 - 2.742
R-factor	0.1781
Rwork	0.175
R-free	0.22160
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.218
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.9_1678))

Data quality characteristics

	Overall
Low resolution limit [Å]	17.879
High resolution limit [Å]	2.742
Number of reflections	32553
Completeness [%]	100.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	292	0.1M HEPES, 8% (w/v) PEG 8000, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 292K