4QIW

Crystal structure of euryarchaeal RNA polymerase from Thermococcus kodakarensis

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, I	DNA-directed RNA polymerase	polymer	906	103038.6	2	UniProt (Q5JE33) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) In PDB	Thermococcus kodakarensis
2	B, J	DNA-directed RNA polymerase	polymer	1123	127468.0	2	UniProt (Q5JE32) Pfam (PF04567) Pfam (PF00562) Pfam (PF04560) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF04566) In PDB	Thermococcus kodakarensis
3	C, M	DNA-directed RNA polymerase subunit A''	polymer	391	43727.4	2	UniProt (Q5JE34) Pfam (PF04998) In PDB	Thermococcus kodakarensis
4	D, O	DNA-directed RNA polymerase subunit D	polymer	259	29429.6	2	UniProt (Q5JJF4) Pfam (PF01193) Pfam (PF01000) In PDB	Thermococcus kodakarensis
5	E, Q	DNA-directed RNA polymerase, subunit E'	polymer	190	21893.4	2	UniProt (Q5JIY4) Pfam (PF03876) Pfam (PF00575) In PDB	Thermococcus kodakarensis
6	F, R	DNA-directed RNA polymerase, subunit F	polymer	122	14519.7	2	UniProt (Q5JI52) Pfam (PF03874) In PDB	Thermococcus kodakarensis
7	H, S	DNA-directed RNA polymerase subunit H	polymer	82	9522.0	2	UniProt (Q5JE31) Pfam (PF01191) In PDB	Thermococcus kodakarensis
8	K, T	DNA-directed RNA polymerase subunit K	polymer	57	6286.5	2	UniProt (Q5JJD0) Pfam (PF01192) In PDB	Thermococcus kodakarensis
9	L, U	DNA-directed RNA polymerase subunit L	polymer	100	11842.4	2	UniProt (Q5JE88) Pfam (PF13656) In PDB	Thermococcus kodakarensis
10	N, V	DNA-directed RNA polymerase subunit N	polymer	65	7602.0	2	UniProt (Q5JJC9) Pfam (PF01194) In PDB	Thermococcus kodakarensis
11	P, W	DNA-directed RNA polymerase subunit P	polymer	49	5553.7	2	UniProt (Q5JDM8) Pfam (PF03604) In PDB	Thermococcus kodakarensis
12	I, A	MAGNESIUM ION	non-polymer		24.3	4	Chemie (MG)
13	B, N, P, I, J...	ZINC ION	non-polymer		65.4	10	Chemie (ZN)
14		water	water		18.0	6	Chemie (HOH)

Sequence modifications

F, R: 1 - 114 (UniProt: Q5JI52)

PDB	External Database	Details
Ile 115	-	EXPRESSION TAG
Asp 116	-	EXPRESSION TAG
Glu 117	-	EXPRESSION TAG
Tyr 118	-	EXPRESSION TAG
Arg 119	-	EXPRESSION TAG
Pro 120	-	EXPRESSION TAG
Leu 121	-	EXPRESSION TAG
Glu 122	-	EXPRESSION TAG

L, U: 1 - 94 (UniProt: Q5JE88)

PDB	External Database	Details
His 95	-	EXPRESSION TAG
His 96	-	EXPRESSION TAG
His 97	-	EXPRESSION TAG
His 98	-	EXPRESSION TAG
His 99	-	EXPRESSION TAG
His 100	-	EXPRESSION TAG

Contents of the asymmetric unit

Polymers	Number of chains	22
	Total formula weight	761766.9
Non-Polymers*	Number of molecules	14
	Total formula weight	751.3
All*	Total formula weight	762518.2

*Water molecules are not included.