4QIW
Crystal structure of euryarchaeal RNA polymerase from Thermococcus kodakarensis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F1 |
Synchrotron site | CHESS |
Beamline | F1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-11-12 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.9179 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 112.975, 206.606, 365.157 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.732 - 3.500 |
R-factor | 0.2786 |
Rwork | 0.277 |
R-free | 0.31560 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 0.962 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHENIX |
Refinement software | PHENIX ((phenix.refine: 1.8.3_1479)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.590 |
High resolution limit [Å] | 3.500 | 3.500 |
Number of reflections | 113257 | |
Completeness [%] | 94.9 | 86.1 |
Redundancy | 4.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 291 | 0.1 M imidazole (pH 8.0), 0.2 M CaCl2, 0.2M NaNO3 and 12% PEG8000, VAPOR DIFFUSION, HANGING DROP, temperature 291K |