4QIW
Crystal structure of euryarchaeal RNA polymerase from Thermococcus kodakarensis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-11-12 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.9179 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 112.975, 206.606, 365.157 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.732 - 3.500 |
| R-factor | 0.2786 |
| Rwork | 0.277 |
| R-free | 0.31560 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.962 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.8.3_1479)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.590 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Number of reflections | 113257 | |
| Completeness [%] | 94.9 | 86.1 |
| Redundancy | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 291 | 0.1 M imidazole (pH 8.0), 0.2 M CaCl2, 0.2M NaNO3 and 12% PEG8000, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
