4QIW

Crystal structure of euryarchaeal RNA polymerase from Thermococcus kodakarensis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Temperature [K]	100
Detector technology	CCD
Collection date	2012-11-12
Detector	ADSC QUANTUM 270
Wavelength(s)	0.9179
Spacegroup name	P 21 21 21
Unit cell lengths	112.975, 206.606, 365.157
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.732 - 3.500
R-factor	0.2786
Rwork	0.277
R-free	0.31560
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.962
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.8.3_1479))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.590
High resolution limit [Å]	3.500	3.500
Number of reflections	113257
Completeness [%]	94.9	86.1
Redundancy		4.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	291	0.1 M imidazole (pH 8.0), 0.2 M CaCl2, 0.2M NaNO3 and 12% PEG8000, VAPOR DIFFUSION, HANGING DROP, temperature 291K