4QF4
Crystal structure of Staphylococcal nuclease variant V23M at cryogenic temperature
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Thermonuclease | polymer | 149 | 16875.4 | 2 | UniProt (P00644) | Staphylococcus aureus | TNase, Micrococcal nuclease, Staphylococcal nuclease, Nuclease B, Nuclease A |
| 2 | C, F (A, B) | THYMIDINE-3',5'-DIPHOSPHATE | non-polymer | 402.2 | 2 | Chemie (THP) PubChem (25021187) PubChem (25113162) PubChem (57299363) PubChem (1618) PubChem (121976) | |||
| 3 | D, G (A, B) | CALCIUM ION | non-polymer | 40.1 | 2 | Chemie (CA) PubChem (271) | |||
| 4 | E, H (A, B) | PHOSPHATE ION | non-polymer | 95.0 | 2 | Chemie (PO4) PubChem (1061) | |||
| 5 | I, J (A, B) | water | water | 18.0 | 113 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 149 (UniProt: P00644)
| PDB | External Database | Details |
|---|---|---|
| Met 23 | Val 105 | engineered mutation |
| Gly 48 | Lys 130 | conflict |
| Lys 50 | Gly 132 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 33750.8 | |
| Non-Polymers* | Number of molecules | 6 |
| Total formula weight | 1074.5 | |
| All* | Total formula weight | 34825.3 |
