4Q5E

Shigella Effector Kinase OspG bound to E2-Ub UbcH7-Ub Conjugate

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Protein kinase OspG	polymer	174	20116.5	1	UniProt (Q3YTH2) In PDB	Shigella sonnei Ss046	Effector protein OspG
2	B	Polyubiquitin	polymer	76	8642.9	1	UniProt (P0CG63) Pfam (PF00240) In PDB	Saccharomyces cerevisiae (Baker's yeast)	Ubiquitin
3	C	Ubiquitin-conjugating enzyme E2 L3	polymer	156	18052.7	1	UniProt (P68036) Pfam (PF00179) In PDB	Homo sapiens (human)	L-UBC, UbcH7, Ubiquitin carrier protein L3, Ubiquitin-conjugating enzyme E2-F1, Ubiquitin-protein ligase L3
4		water	water		18.0	237	Chemie (HOH)

Sequence modifications

A: 26 - 196 (UniProt: Q3YTH2)

PDB	External Database	Details
Ser 23	-	EXPRESSION TAG
Asn 24	-	EXPRESSION TAG
Ala 25	-	EXPRESSION TAG

B: 1 - 76 (UniProt: P0CG63)

PDB	External Database	Details
Arg 48	Lys 48	ENGINEERED MUTATION
Cys 76	Gly 76	ENGINEERED MUTATION

C: 1 - 154 (UniProt: P68036)

PDB	External Database	Details
Gly -1	-	EXPRESSION TAG
His 0	-	EXPRESSION TAG
Ser 17	Cys 17	ENGINEERED MUTATION
Ser 137	Cys 137	ENGINEERED MUTATION

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	46812.1
All*	Total formula weight	46812.1

*Water molecules are not included.