4PWX

Crystal structure of an ATP-bound Get3-Get4-Get5 complex from S.cerevisiae

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	ATPase GET3	polymer	356	39547.7	2	UniProt (Q12154) Pfam (PF02374)	Saccharomyces cerevisiae (Baker's yeast)	Arsenical pump-driving ATPase, Arsenite-stimulated ATPase, Golgi to ER traffic protein 3, Guided entry of tail-anchored proteins 3
2	C, E (E, C)	Golgi to ER traffic protein 4	polymer	288	33806.1	2	UniProt (Q12125) Pfam (PF04190)	Saccharomyces cerevisiae (Baker's yeast)	Guided entry of tail-anchored proteins 4
3	D, F (F, D)	Ubiquitin-like protein MDY2	polymer	54	6034.0	2	UniProt (Q12285) Pfam (PF16843)	Saccharomyces cerevisiae (Baker's yeast)	Golgi to ER traffic protein 5, Mating-deficient protein 2, Translation machinery-associated protein 24
4	G, J (A, B)	ADENOSINE-5'-TRIPHOSPHATE	non-polymer		507.2	2	Chemie (ATP)
5	H, K (A, B)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
6	I (A)	ZINC ION	non-polymer		65.4	1	Chemie (ZN)

Sequence modifications

A, B: 2 - 354 (UniProt: Q12154)

PDB	External Database	Details
Ser -1	-	expression tag
Val 0	-	expression tag
Asp 1	-	expression tag
Val 57	Asp 57	engineered mutation

E, C: 11 - 290 (UniProt: Q12125)

PDB	External Database	Details
Met 9	-	expression tag
Gly 10	-	expression tag
Ala 258	Lys 258	engineered mutation
Ala 260	Lys 260	engineered mutation
Glu 291	-	expression tag
Asn 292	-	expression tag
Leu 293	-	expression tag
Tyr 294	-	expression tag
Phe 295	-	expression tag
Gln 296	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	158775.8
Non-Polymers*	Number of molecules	5
	Total formula weight	1128.4
All*	Total formula weight	159904.2

*Water molecules are not included.