4PWX
Crystal structure of an ATP-bound Get3-Get4-Get5 complex from S.cerevisiae
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | OTHER |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-02-22 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.0 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 166.341, 134.501, 84.060 |
Unit cell angles | 90.00, 113.37, 90.00 |
Refinement procedure
Resolution | 30.000 - 5.400 |
Rwork | 0.270 |
R-free | 0.32800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB ENTRIES 2WOJ AND 3LKU |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (v6.3) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 6.000 |
High resolution limit [Å] | 5.400 | 5.400 |
Rmerge | 0.050 | 0.460 |
Number of reflections | 5529 | |
<I/σ(I)> | 2 | |
Completeness [%] | 93.8 | 95.9 |
Redundancy | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 9 | 298 | 18% PEG 3350, 0.2M potassium thiocyanate, 0.1M bis-tris propane, 5% DMSO, pH 9.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |