4PQT
Insights into the mechanism of deubiquitination by JAMM deubiquitinases from co-crystal structures of enzyme with substrate and product
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | AMSH-like protease sst2 | polymer | 197 | 21937.7 | 1 | UniProt (Q9P371) Pfam (PF01398) | Schizosaccharomyces pombe (Fission yeast) | Suppressor of ste12 deletion protein 2 |
| 2 | B (B) | Protein UBBP4 | polymer | 81 | 8988.3 | 1 | UniProt (J3QRK5) | Homo sapiens (human) | |
| 3 | C (A) | ZINC ION | non-polymer | 65.4 | 1 | Chemie (ZN) | |||
| 4 | D, E, F (A, B) | 1,2-ETHANEDIOL | non-polymer | 62.1 | 3 | Chemie (EDO) | |||
| 5 | G, H (A, B) | water | water | 18.0 | 78 | Chemie (HOH) |
Sequence modifications
A: 245 - 435 (UniProt: Q9P371)
B: 1 - 76 (UniProt: J3QRK5)
| PDB | External Database | Details |
|---|---|---|
| Gly 239 | - | expression tag |
| Pro 240 | - | expression tag |
| Leu 241 | - | expression tag |
| Gly 242 | - | expression tag |
| Ser 243 | - | expression tag |
| Met 244 | - | expression tag |
| Ala 354 | Asp 354 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Gly -4 | - | expression tag |
| Pro -3 | - | expression tag |
| Leu -2 | - | expression tag |
| Gly -1 | - | expression tag |
| Ser 0 | - | expression tag |
| Thr 55 | Ser 131 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 30926.0 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 251.6 | |
| All* | Total formula weight | 31177.6 |
