4PIP
Engineered EgtD variant EgtD-M252V,E282A in complex with tryptophan and SAH
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D (A, B, C, D) | Histidine-specific methyltransferase EgtD | polymer | 323 | 35192.6 | 4 | UniProt (A0R5M8) Pfam (PF10017) | Mycobacterium smegmatis | Histidine-alpha-N-methyltransferase |
| 2 | E, G, K, N (A, B, C, D) | TRYPTOPHAN | non-polymer | 204.2 | 4 | Chemie (TRP) | |||
| 3 | F, H, L, O (A, B, C, D) | S-ADENOSYL-L-HOMOCYSTEINE | non-polymer | 384.4 | 4 | Chemie (SAH) | |||
| 4 | I, M (B, C) | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) | |||
| 5 | J (C) | CHLORIDE ION | non-polymer | 35.5 | 1 | Chemie (CL) | |||
| 6 | P, Q, R, S (A, B, C, D) | water | water | 18.0 | 1369 | Chemie (HOH) |
Sequence modifications
A, B, C, D: 1 - 321 (UniProt: A0R5M8)
| PDB | External Database | Details |
|---|---|---|
| Gly -1 | - | expression tag |
| His 0 | - | expression tag |
| Ala 2 | Thr 2 | engineered mutation |
| Thr 29 | Ala 29 | engineered mutation |
| Gln 30 | Pro 30 | engineered mutation |
| Ser 75 | Ala 75 | engineered mutation |
| Val 252 | Met 252 | engineered mutation |
| Ala 282 | Glu 282 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 140770.4 | |
| Non-Polymers* | Number of molecules | 11 |
| Total formula weight | 2438.6 | |
| All* | Total formula weight | 143209.0 |
