4PIP

Engineered EgtD variant EgtD-M252V,E282A in complex with tryptophan and SAH

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0008168	molecular_function	methyltransferase activity
A	0008276	molecular_function	protein methyltransferase activity
A	0016740	molecular_function	transferase activity
A	0032259	biological_process	methylation
A	0052699	biological_process	ergothioneine biosynthetic process
A	0052704	biological_process	ergothioneine biosynthesis from histidine via gamma-glutamyl-hercynylcysteine sulfoxide
A	0052706	molecular_function	L-histidine N(alpha)-methyltransferase activity
B	0008168	molecular_function	methyltransferase activity
B	0008276	molecular_function	protein methyltransferase activity
B	0016740	molecular_function	transferase activity
B	0032259	biological_process	methylation
B	0052699	biological_process	ergothioneine biosynthetic process
B	0052704	biological_process	ergothioneine biosynthesis from histidine via gamma-glutamyl-hercynylcysteine sulfoxide
B	0052706	molecular_function	L-histidine N(alpha)-methyltransferase activity
C	0008168	molecular_function	methyltransferase activity
C	0008276	molecular_function	protein methyltransferase activity
C	0016740	molecular_function	transferase activity
C	0032259	biological_process	methylation
C	0052699	biological_process	ergothioneine biosynthetic process
C	0052704	biological_process	ergothioneine biosynthesis from histidine via gamma-glutamyl-hercynylcysteine sulfoxide
C	0052706	molecular_function	L-histidine N(alpha)-methyltransferase activity
D	0008168	molecular_function	methyltransferase activity
D	0008276	molecular_function	protein methyltransferase activity
D	0016740	molecular_function	transferase activity
D	0032259	biological_process	methylation
D	0052699	biological_process	ergothioneine biosynthetic process
D	0052704	biological_process	ergothioneine biosynthesis from histidine via gamma-glutamyl-hercynylcysteine sulfoxide
D	0052706	molecular_function	L-histidine N(alpha)-methyltransferase activity

Functional Information from PDB Data

site_id	AC1
Number of Residues	10
Details	binding site for residue TRP A 401

Chain	Residue
A	TYR39
A	HOH743
A	PHE47
A	TYR56
A	GLY161
A	ASN166
A	TYR206
A	ALA282
A	SER284
A	HOH633

---

site_id	AC2
Number of Residues	21
Details	binding site for residue SAH A 402

Chain	Residue
A	LYS36
A	TYR39
A	GLU84
A	GLY86
A	SER87
A	THR89
A	LYS92
A	ASP113
A	VAL114
A	GLY140
A	ASP141
A	PHE142
A	LEU160
A	GLY161
A	HOH635
A	HOH640
A	HOH642
A	HOH649
A	HOH690
A	HOH693
A	HOH699

---

site_id	AC3
Number of Residues	10
Details	binding site for residue TRP B 401

Chain	Residue
B	TYR39
B	TYR56
B	GLY161
B	ASN166
B	TYR206
B	ALA282
B	SER284
B	HOH741
B	HOH745
B	HOH756

---

site_id	AC4
Number of Residues	21
Details	binding site for residue SAH B 402

Chain	Residue
B	LYS36
B	TYR39
B	PHE47
B	GLY86
B	SER87
B	LYS92
B	ASP113
B	VAL114
B	GLY140
B	ASP141
B	PHE142
B	LEU160
B	GLY161
B	HOH623
B	HOH629
B	HOH657
B	HOH665
B	HOH683
B	HOH703
B	HOH720
B	HOH757

---

site_id	AC5
Number of Residues	6
Details	binding site for residue MG B 403

Chain	Residue
B	HOH522
B	HOH539
B	HOH553
C	HOH529
C	HOH546
C	HOH577

---

site_id	AC6
Number of Residues	4
Details	binding site for residue CL C 401

Chain	Residue
C	GLY-1
C	HIS0
C	HOH592
D	HIS305

---

site_id	AC7
Number of Residues	11
Details	binding site for residue TRP C 402

Chain	Residue
C	PHE47
C	TYR56
C	GLY161
C	THR163
C	ASN166
C	TYR206
C	PHE216
C	ALA282
C	SER284
C	HOH750
C	HOH805

---

site_id	AC8
Number of Residues	20
Details	binding site for residue SAH C 403

Chain	Residue
C	HOH658
C	HOH662
C	HOH710
C	HOH822
C	LYS36
C	TYR39
C	PHE47
C	GLY86
C	SER87
C	GLY88
C	LYS92
C	ASP113
C	VAL114
C	GLY140
C	ASP141
C	PHE142
C	GLY161
C	THR163
C	HOH634
C	HOH647

---

site_id	AC9
Number of Residues	6
Details	binding site for residue MG C 404

Chain	Residue
B	HOH524
B	HOH542
B	HOH583
C	HOH531
C	HOH572
D	HOH520

---

site_id	AD1
Number of Residues	11
Details	binding site for residue TRP D 401

Chain	Residue
D	PHE38
D	PHE47
D	TYR56
D	GLY161
D	THR163
D	ASN166
D	TYR206
D	THR213
D	ALA282
D	SER284
D	HOH633

---

site_id	AD2
Number of Residues	19
Details	binding site for residue SAH D 402

Chain	Residue
D	TYR39
D	PHE47
D	GLY86
D	SER87
D	GLY88
D	LYS92
D	ASP113
D	VAL114
D	GLY140
D	ASP141
D	PHE142
D	GLY161
D	THR163
D	HOH649
D	HOH657
D	HOH669
D	HOH673
D	HOH710
D	HOH797

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	20
Details	Binding site: {"evidences":[{"source":"PubMed","id":"25251321","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"25404173","evidenceCode":"ECO:0000305"},{"source":"PDB","id":"4UY6","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4UY7","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	12
Details	Binding site: {"evidences":[{"source":"PubMed","id":"25251321","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"25404173","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	4
Details	Binding site: {"evidences":[{"source":"PubMed","id":"25251321","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"25404173","evidenceCode":"ECO:0000305"},{"source":"PDB","id":"4PIO","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4PIP","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4UY6","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

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