4PEU

Structure of the polysaccharide lyase-like protein Cthe_2159 from C. thermocellum, native form with Calcium bound

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Uncharacterized protein	polymer	313	32859.8	1	UniProt (A3DHD2) Pfam (PF14262) In PDB	Clostridium thermocellum
2	A	CALCIUM ION	non-polymer		40.1	3	Chemie (CA)
3		water	water		18.0	403	Chemie (HOH)

Sequence modifications

A: 2 - 286 (UniProt: A3DHD2)

PDB	External Database	Details
Met -4	-	expression tag
Gly -3	-	expression tag
Ala -2	-	expression tag
His -1	-	expression tag
Ala 0	-	expression tag
Val 1	-	expression tag
Gly 199	Asp 199	engineered mutation
Gly 213	Glu 213	engineered mutation
Ala 287	-	expression tag
Ser 288	-	expression tag
Gly 289	-	expression tag
Lys 290	-	expression tag
Pro 291	-	expression tag
Ile 292	-	expression tag
Pro 293	-	expression tag
Asn 294	-	expression tag
Pro 295	-	expression tag
Leu 296	-	expression tag
Leu 297	-	expression tag
Gly 298	-	expression tag
Leu 299	-	expression tag
Asp 300	-	expression tag
Ser 301	-	expression tag
Thr 302	-	expression tag
His 303	-	expression tag
His 304	-	expression tag
His 305	-	expression tag
His 306	-	expression tag
His 307	-	expression tag
His 308	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	32859.8
Non-Polymers*	Number of molecules	3
	Total formula weight	120.2
All*	Total formula weight	32980.0

*Water molecules are not included.