4PEU

Structure of the polysaccharide lyase-like protein Cthe_2159 from C. thermocellum, native form with Calcium bound

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2011-09-09
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 21 21 2
Unit cell lengths	70.700, 123.429, 34.530
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.800 - 1.800
R-factor	0.148521419908
Rwork	0.146
R-free	0.17103
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	1.078
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.9_1685)

Data quality characteristics

	Overall
Low resolution limit [Å]	26.800
High resolution limit [Å]	1.800
Rmerge	0.026
Number of reflections	28069
<I/σ(I)>	17.16
Completeness [%]	97.2
Redundancy	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		295	19% PEG 8000, 0.1M MES pH 6.0, and 0.1M Calcium acetate