4PAB

Crystal structure of the precursor form of rat DMGDH complexed with tetrahydrofolate

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Dimethylglycine dehydrogenase	polymer	869	97540.9	2	UniProt (Q5RKL4) Pfam (PF01266) Pfam (PF16350) Pfam (PF01571) Pfam (PF08669) UniProt (by SIFTS) (Q63342) In PDB	Rattus norvegicus (Rat)	Dimethylglycine dehydrogenase,mitochondrial
2	A, B	FLAVIN-ADENINE DINUCLEOTIDE	non-polymer		785.5	2	Chemie (FAD)
3	A, B	(6S)-5,6,7,8-TETRAHYDROFOLATE	non-polymer		445.4	2	Chemie (THG)
4	A, B	THIOCYANATE ION	non-polymer		58.1	8	Chemie (SCN)
5		water	water		18.0	721	Chemie (HOH)

Sequence modifications

A, B: 2 - 857 (UniProt: Q5RKL4)

PDB	External Database	Details
Met -1	-	expression tag
Ala 0	-	expression tag
Ser 1	-	expression tag
Val 204	Ala 204	engineered mutation
Val 267	Ile 267	engineered mutation
Lys 402	Thr 402	engineered mutation
Arg 509	Glu 509	engineered mutation
Asp 746	Asn 746	engineered mutation
Asp 774	Asn 774	engineered mutation
Ala 858	-	expression tag
Ala 859	-	expression tag
Leu 860	-	expression tag
Glu 861	-	expression tag
His 862	-	expression tag
His 863	-	expression tag
His 864	-	expression tag
His 865	-	expression tag
His 866	-	expression tag
His 867	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	195081.7
Non-Polymers*	Number of molecules	12
	Total formula weight	2926.6
All*	Total formula weight	198008.3

*Water molecules are not included.