4P6Z

Crystal structure of the human BST2 cytoplasmic domain and the HIV-1 Vpu cytoplasmic domain bound to the clathrin adaptor protein complex 1 (AP1) core

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (G)	AP-1 complex subunit gamma-1	polymer	627	70944.0	1	UniProt (P22892) Pfam (PF01602)	Mus musculus (Mouse)	Adapter-related protein complex 1 subunit gamma-1,Adaptor protein complex AP-1 subunit gamma-1,Clathrin assembly protein complex 1 gamma-1 large chain,Gamma-adaptin,Gamma1-adaptin,Golgi adaptor HA1/AP1 adaptin subunit gamma-1
2	B (S)	AP-1 complex subunit sigma-1A	polymer	158	18787.1	1	UniProt (P61966) Pfam (PF01217)	Homo sapiens (Human)	Adapter-related protein complex 1 subunit sigma-1A,Adaptor protein complex AP-1 subunit sigma-1A,Clathrin assembly protein complex 1 sigma-1A small chain,Clathrin coat assembly protein AP19,Golgi adaptor HA1/AP1 adaptin sigma-1A subunit,HA1 19 kDa subunit,Sigma 1a subunit of AP-1 clathrin,Sigma-adaptin 1A,Sigma1A-adaptin
3	C (M)	AP-1 complex subunit mu-1	polymer	423	48606.7	1	UniProt (P35585) Pfam (PF01217) Pfam (PF00928)	Mus musculus (Mouse)	AP-mu chain family member mu1A,Adapter-related protein complex 1 subunit mu-1,Adaptor protein complex AP-1 subunit mu-1,Clathrin assembly protein complex 1 mu-1 medium chain 1,Clathrin coat assembly protein AP47,Clathrin coat-associated protein AP47,Golgi adaptor HA1/AP1 adaptin mu-1 subunit,Mu-adaptin 1,Mu1A-adaptin
4	D (B)	AP-1 complex subunit beta-1	polymer	600	67770.3	1	UniProt (Q10567) Pfam (PF01602)	Homo sapiens (Human)	Adapter-related protein complex 1 subunit beta-1,Adaptor protein complex AP-1 subunit beta-1,Beta-1-adaptin,Beta-adaptin 1,Clathrin assembly protein complex 1 beta large chain,Golgi adaptor HA1/AP1 adaptin beta subunit
5	E (V)	Protein Vpu	polymer	63	7123.6	1	UniProt (P19554)	Human immunodeficiency virus type 1 group M subtype B (HIV-1)	U ORF protein,Viral protein U
6	F (T)	Bone marrow stromal antigen 2	polymer	25	2822.2	1	UniProt (Q10589)	Homo sapiens (Human)	BST-2,HM1.24 antigen,Tetherin
7	G, H, I, J (G, S, M, B)	water	water		18.0	14	Chemie (HOH)

Sequence modifications

G: 1 - 613 (UniProt: P22892)

PDB	External Database	Details
Met -13	-	initiating methionine
Gly -12	-	expression tag
Ser -11	-	expression tag
Ser -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
Ser -3	-	expression tag
Gln -2	-	expression tag
Asp -1	-	expression tag
Pro 0	-	expression tag

S: 1 - 158 (UniProt: P61966)

PDB	External Database	Details
Arg 11	Gln 11	engineered mutation

B: 1 - 584 (UniProt: Q10567)

PDB	External Database	Details
Met -15	-	initiating methionine
Gly -14	-	expression tag
Ser -13	-	expression tag
Ser -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
Ser -5	-	expression tag
Gln -4	-	expression tag
Asp -3	-	expression tag
Pro -2	-	expression tag
Asn -1	-	expression tag
Ser 0	-	expression tag
Ala 431	Thr 431	engineered mutation
Lys 476	Glu 476	engineered mutation

V: 28 - 80 (UniProt: P19554)

PDB	External Database	Details
Gly 18	-	expression tag
Ser 19	-	expression tag
Asp 20	-	expression tag
Glu 21	-	expression tag
Ala 22	-	expression tag
Ser 23	-	expression tag
Glu 24	-	expression tag
Gly 25	-	expression tag
Ser 26	-	expression tag
Gly 27	-	expression tag

T: 1 - 21 (UniProt: Q10589)

PDB	External Database	Details
Ala -3	-	expression tag
Gly -2	-	expression tag
Phe -1	-	expression tag
Ser 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	216053.9
All*	Total formula weight	216053.9

*Water molecules are not included.