4P1Y

Crystal structure of staphylococcal gamma-hemolysin prepore

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C, E, G	Gamma-hemolysin component B	polymer	309	35054.8	4	UniProt (Q931F3) Pfam (PF07968) UniProt (by SIFTS) (A0A0H3JX61) In PDB	Staphylococcus aureus	LukF
2	B, D, F, H	Gamma-hemolysin component A	polymer	290	33186.4	4	UniProt (P0A071) Pfam (PF07968) In PDB	Staphylococcus aureus	H-gamma-2, H-gamma-II, Hlg2

Sequence modifications

A, C, E, G: 2 - 300 (UniProt: Q931F3)

PDB	External Database	Details
Met -8	-	expression tag
Gly -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
Ala 0	-	expression tag
Met 1	-	expression tag
Ala 177	Trp 202	engineered mutation
Ala 198	Arg 223	engineered mutation

B, D, F, H: 13 - 280 (UniProt: P0A071)

PDB	External Database	Details
Met -9	-	expression tag
Gly -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
Ala -1	-	expression tag
Met 0	-	expression tag
Glu 1	-	expression tag
Asn 2	-	expression tag
Lys 3	-	expression tag
Ile 4	-	expression tag
Glu 5	-	expression tag
Asp 6	-	expression tag
Ile 7	-	expression tag
Gly 8	-	expression tag
Gln 9	-	expression tag
Gly 10	-	expression tag
Ala 11	-	expression tag
Glu 12	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	272964.7
All*	Total formula weight	272964.7

*Water molecules are not included.