4P1Y
Crystal structure of staphylococcal gamma-hemolysin prepore
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-1A |
Synchrotron site | Photon Factory |
Beamline | BL-1A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-12-13 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 1 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 197.056, 197.050, 192.965 |
Unit cell angles | 90.00, 99.04, 90.00 |
Refinement procedure
Resolution | 44.399 - 2.992 |
R-factor | 0.2312 |
Rwork | 0.230 |
R-free | 0.25430 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.037 |
Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.400 | 3.099 |
High resolution limit [Å] | 2.992 | 2.992 |
Rmerge | 0.186 | 0.622 |
Number of reflections | 139696 | |
<I/σ(I)> | 4.73 | 1.53 |
Completeness [%] | 95.5 | 77.76 |
Redundancy | 3.3 | 2.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.8 | 298 | 60% MPD, 0.1M sodium acetate (pH 4.8), 0.15M di-sodium hydrogen phosphate |