4OY4
calcium-free CaMPARI v0.2
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Chimera protein of Calmodulin, GPF-like protein EosFP, and Myosin light chain kinase | polymer | 415 | 47289.0 | 1 | UniProt (P62161) UniProt (Q5S6Z9) UniProt (Q6LDG3) Pfam (PF13499) Pfam (PF01353) UniProt (by SIFTS) (P0DP29) UniProt (by SIFTS) (P11799) | Rattus norvegicus (Rat) | CaM |
| 2 | B, C (A) | SULFATE ION | non-polymer | 96.1 | 2 | Chemie (SO4) | |||
| 3 | D, E (A) | GLYCEROL | non-polymer | 92.1 | 2 | Chemie (GOL) | |||
| 4 | F (A) | water | water | 18.0 | 104 | Chemie (HOH) |
Sequence modifications
A: 1 - 145 (UniProt: P62161)
A: 151 - 231 (UniProt: Q5S6Z9)
A: 242 - 382 (UniProt: Q5S6Z9)
A: 388 - 406 (UniProt: Q6LDG3)
| PDB | External Database | Details |
|---|---|---|
| Met -8 | - | expression tag |
| His -7 | - | expression tag |
| His -6 | - | expression tag |
| His -5 | - | expression tag |
| His -4 | - | expression tag |
| His -3 | - | expression tag |
| His -2 | - | expression tag |
| Gly -1 | - | expression tag |
| Ser 0 | - | expression tag |
| Asp 59 | Asn 61 | engineered mutation |
| Ala 146 | - | linker |
| Lys 147 | - | linker |
| Leu 148 | - | linker |
| Glu 149 | - | linker |
| Cys 150 | - | linker |
| PDB | External Database | Details |
|---|---|---|
| His 165 | Thr 158 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Arg 232 | - | linker |
| Arg 233 | - | linker |
| Gly 234 | - | linker |
| Gly 235 | - | linker |
| Thr 236 | - | linker |
| Gly 237 | - | linker |
| Gly 238 | - | linker |
| Ser 239 | - | linker |
| Met 240 | - | linker |
| Val 241 | - | linker |
| Lys 251 | Asn 11 | engineered mutation |
| Tyr 274 | Phe 34 | engineered mutation |
| Thr 279 | Ser 39 | engineered mutation |
| Cr8 303 | His 62 | chromophore |
| Cr8 303 | Tyr 63 | chromophore |
| Cr8 303 | Gly 64 | chromophore |
| Val 309 | Ala 69 | engineered mutation |
| Lys 310 | Glu 70 | engineered mutation |
| Asn 314 | His 74 | engineered mutation |
| Met 333 | Leu 93 | engineered mutation |
| Tyr 342 | Ile 102 | engineered mutation |
| Tyr 361 | His 121 | engineered mutation |
| Thr 363 | Val 123 | engineered mutation |
| Met 380 | Glu 140 | engineered mutation |
| Trp 383 | - | linker |
| Thr 384 | - | linker |
| Arg 385 | - | linker |
| Ser 386 | - | linker |
| Ser 387 | - | linker |
| PDB | External Database | Details |
|---|---|---|
| Asn 392 | Gln 41 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 47289.0 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 376.3 | |
| All* | Total formula weight | 47665.3 |
