4OY4

calcium-free CaMPARI v0.2

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Chimera protein of Calmodulin, GPF-like protein EosFP, and Myosin light chain kinase	polymer	415	47289.0	1	UniProt (P62161) UniProt (Q5S6Z9) UniProt (Q6LDG3) Pfam (PF13499) Pfam (PF01353) UniProt (by SIFTS) (P0DP29) UniProt (by SIFTS) (P11799) In PDB	Rattus norvegicus (Rat)	CaM
2	A	SULFATE ION	non-polymer		96.1	2	Chemie (SO4)
3	A	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
4		water	water		18.0	104	Chemie (HOH)

Sequence modifications

A: 1 - 145 (UniProt: P62161)

PDB	External Database	Details
Met -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Asp 59	Asn 61	engineered mutation
Ala 146	-	linker
Lys 147	-	linker
Leu 148	-	linker
Glu 149	-	linker
Cys 150	-	linker

A: 151 - 231 (UniProt: Q5S6Z9)

PDB	External Database	Details
His 165	Thr 158	engineered mutation

A: 242 - 382 (UniProt: Q5S6Z9)

PDB	External Database	Details
Arg 232	-	linker
Arg 233	-	linker
Gly 234	-	linker
Gly 235	-	linker
Thr 236	-	linker
Gly 237	-	linker
Gly 238	-	linker
Ser 239	-	linker
Met 240	-	linker
Val 241	-	linker
Lys 251	Asn 11	engineered mutation
Tyr 274	Phe 34	engineered mutation
Thr 279	Ser 39	engineered mutation
Cr8 303	His 62	chromophore
Cr8 303	Tyr 63	chromophore
Cr8 303	Gly 64	chromophore
Val 309	Ala 69	engineered mutation
Lys 310	Glu 70	engineered mutation
Asn 314	His 74	engineered mutation
Met 333	Leu 93	engineered mutation
Tyr 342	Ile 102	engineered mutation
Tyr 361	His 121	engineered mutation
Thr 363	Val 123	engineered mutation
Met 380	Glu 140	engineered mutation
Trp 383	-	linker
Thr 384	-	linker
Arg 385	-	linker
Ser 386	-	linker
Ser 387	-	linker

A: 388 - 406 (UniProt: Q6LDG3)

PDB	External Database	Details
Asn 392	Gln 41	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	47289.0
Non-Polymers*	Number of molecules	4
	Total formula weight	376.3
All*	Total formula weight	47665.3

*Water molecules are not included.