4OY4
calcium-free CaMPARI v0.2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-11-16 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 68.670, 68.670, 172.770 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 53.750 - 2.030 |
R-factor | 0.1981 |
Rwork | 0.196 |
R-free | 0.23519 |
RMSD bond length | 0.019 |
RMSD bond angle | 2.006 |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 57.590 |
High resolution limit [Å] | 2.030 |
Number of reflections | 27700 |
<I/σ(I)> | 13.4 |
Completeness [%] | 99.9 |
Redundancy | 7.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277.15 | 0.2 M Ammonium sulfate, 0.1 M HEPES pH 7.5, 25% w/v Polyethylene glycol 3,350, 10 mM EGTA |