4OY4

calcium-free CaMPARI v0.2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2012-11-16
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	P 43 21 2
Unit cell lengths	68.670, 68.670, 172.770
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	53.750 - 2.030
R-factor	0.1981
Rwork	0.196
R-free	0.23519
RMSD bond length	0.019
RMSD bond angle	2.006
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall
Low resolution limit [Å]	57.590
High resolution limit [Å]	2.030
Number of reflections	27700
<I/σ(I)>	13.4
Completeness [%]	99.9
Redundancy	7.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277.15	0.2 M Ammonium sulfate, 0.1 M HEPES pH 7.5, 25% w/v Polyethylene glycol 3,350, 10 mM EGTA