4ORN

Blue Fluorescent Protein mKalama1

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	mKalama1	polymer	241	27014.6	2	UniProt (P42212) Pfam (PF01353) In PDB	Aequorea victoria (Jellyfish)
2	A	2-(N-MORPHOLINO)-ETHANESULFONIC ACID	non-polymer		195.2	2	Chemie (MES)
3	A, B	SULFATE ION	non-polymer		96.1	6	Chemie (SO4)
4	A, B	CHLORIDE ION	non-polymer		35.5	4	Chemie (CL)
5		water	water		18.0	402	Chemie (HOH)

Sequence modifications

A, B: 2 - 238 (UniProt: P42212)

PDB	External Database	Details
Ser -2	-	expression tag
Ser -1	-	expression tag
Met 0	-	expression tag
Val 1	-	expression tag
Met 18	Leu 18	engineered mutation
Arg 25	His 25	engineered mutation
Arg 30	Ser 30	engineered mutation
Val 32	Glu 32	engineered mutation
His 39	Tyr 39	engineered mutation
Ser 50	Thr 50	engineered mutation
Leu 64	Phe 64	engineered mutation
Gys 66	Ser 65	chromophore
Gys 66	Tyr 66	chromophore
Gys 66	Gly 67	chromophore
Ser 105	Asn 105	engineered mutation
Val 124	Glu 124	engineered mutation
Thr 128	Ile 128	engineered mutation
Met 145	Tyr 145	engineered mutation
Val 147	Ser 147	engineered mutation
Gly 148	His 148	engineered mutation
Thr 153	Met 153	engineered mutation
Ala 163	Val 163	engineered mutation
Glu 166	Lys 166	engineered mutation
Val 171	Ile 171	engineered mutation
Gly 175	Ser 175	engineered mutation
Ser 192	Pro 192	engineered mutation
Val 203	Thr 203	engineered mutation
Val 205	Ser 205	engineered mutation
Lys 206	Ala 206	engineered mutation
Arg 224	Val 224	engineered mutation
Pro 231	His 231	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	54029.1
Non-Polymers*	Number of molecules	12
	Total formula weight	1108.7
All*	Total formula weight	55137.8

*Water molecules are not included.