4ORN

Blue Fluorescent Protein mKalama1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9686
Spacegroup name	P 21 21 21
Unit cell lengths	80.510, 83.460, 92.240
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	60.655 - 1.710
R-factor	0.1594
Rwork	0.158
R-free	0.18330
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2qle
RMSD bond length	0.012
RMSD bond angle	1.673
Data reduction software	MOSFLM
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	80.510		1.750
High resolution limit [Å]	1.710	7.650	1.710
Rmerge	0.069	0.035	0.908
Number of reflections	67617	1459	9510
<I/σ(I)>	14.99	36	2.48
Completeness [%]	99.8	99.5	99.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	294	1.8 M ammonium sulfate, 0.01 M cobalt chloride, 0.1 M MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K