4OO1

Structure of an Rrp6-RNA exosome complex bound to poly(A) RNA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Exosome complex component RRP45	polymer	305	34001.9	1	UniProt (Q05636) Pfam (PF01138) Pfam (PF03725) In PDB	Saccharomyces cerevisiae (Baker's yeast)	Ribosomal RNA-processing protein 45
2	B	Exosome complex component SKI6	polymer	250	28007.1	1	UniProt (P46948) Pfam (PF01138) Pfam (PF03725) In PDB	Saccharomyces cerevisiae (Baker's yeast)	Extracellular mutant protein 20, Ribosomal RNA-processing protein 41, Superkiller protein 6
3	C	Exosome complex component RRP43	polymer	394	44060.9	1	UniProt (P25359) Pfam (PF01138) In PDB	Saccharomyces cerevisiae (Baker's yeast)	Ribosomal RNA-processing protein 43
4	D	Exosome complex component RRP46	polymer	225	24574.3	1	UniProt (P53256) Pfam (PF01138) Pfam (PF03725) In PDB	Saccharomyces cerevisiae (Baker's yeast)	Ribosomal RNA-processing protein 46
5	E	Exosome complex component RRP42	polymer	269	29492.6	1	UniProt (Q12277) In PDB	Saccharomyces cerevisiae (Baker's yeast)	Ribosomal RNA-processing protein 42
6	F	Exosome complex component MTR3	polymer	250	27604.0	1	UniProt (P48240) Pfam (PF01138) In PDB	Saccharomyces cerevisiae (Baker's yeast)	mRNA transport regulator 3
7	G	Exosome complex component RRP40	polymer	244	26990.8	1	UniProt (Q08285) Pfam (PF21262) Pfam (PF15985) Pfam (PF18311) In PDB	Saccharomyces cerevisiae (Baker's yeast)	Ribosomal RNA-processing protein 40
8	H	Exosome complex component RRP4	polymer	363	39877.6	1	UniProt (P38792) Pfam (PF14382) Pfam (PF21266) Pfam (PF15985) In PDB	Saccharomyces cerevisiae (Baker's yeast)	Ribosomal RNA-processing protein 4
9	I	Exosome complex component CSL4	polymer	296	32026.7	1	UniProt (P53859) Pfam (PF21551) Pfam (PF10447) In PDB	Saccharomyces cerevisiae (Baker's yeast)	CEP1 synthetic lethal protein 4
10	J	Exosome complex exonuclease RRP6	polymer	560	64612.7	1	UniProt (Q12149) Pfam (PF01612) Pfam (PF00570) In PDB	Saccharomyces cerevisiae (Baker's yeast)	Ribosomal RNA-processing protein 6
11	S	POLY A RNA	polymer	24	7856.0	1
12	E, S	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
13	E	(4S)-2-METHYL-2,4-PENTANEDIOL	non-polymer		118.2	1	Chemie (MPD)
14	G	2-(N-MORPHOLINO)-ETHANESULFONIC ACID	non-polymer		195.2	1	Chemie (MES)
15	I, S	PHOSPHATE ION	non-polymer		95.0	2	Chemie (PO4)

Sequence modifications

B: 1 - 246 (UniProt: P46948)

PDB	External Database	Details
Gly -3	-	expression tag
Pro -2	-	expression tag
Asp -1	-	expression tag
His 0	-	expression tag

D: 1 - 223 (UniProt: P53256)

PDB	External Database	Details
Gly -1	-	expression tag
Ser 0	-	expression tag

E: 1 - 265 (UniProt: Q12277)

PDB	External Database	Details
Gly -3	-	expression tag
Asp -2	-	expression tag
Pro -1	-	expression tag
His 0	-	expression tag

G: 1 - 240 (UniProt: Q08285)

PDB	External Database	Details
Gly -3	-	expression tag
Asp -2	-	expression tag
Pro -1	-	expression tag
His 0	-	expression tag

H: 1 - 359 (UniProt: P38792)

PDB	External Database	Details
Gly -3	-	expression tag
Asp -2	-	expression tag
Pro -1	-	expression tag
His 0	-	expression tag

I: 1 - 292 (UniProt: P53859)

PDB	External Database	Details
Gly -3	-	expression tag
Asp -2	-	expression tag
Pro -1	-	expression tag
His 0	-	expression tag

J: 129 - 685 (UniProt: Q12149)

PDB	External Database	Details
Ser 126	-	expression tag
Leu 127	-	expression tag
Met 128	-	expression tag
Asn 238	Asp 238	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	11
	Total formula weight	359104.5
Non-Polymers*	Number of molecules	6
	Total formula weight	552.0
All*	Total formula weight	359656.5

*Water molecules are not included.