4OO1
Structure of an Rrp6-RNA exosome complex bound to poly(A) RNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-03-29 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0750 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 193.292, 200.083, 97.405 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.426 - 3.300 |
R-factor | 0.2288 |
Rwork | 0.227 |
R-free | 0.26490 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB ENTRIES 4IFD 2NN6 AND 2HBL |
RMSD bond length | 0.002 |
RMSD bond angle | 0.474 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHENIX |
Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.420 |
High resolution limit [Å] | 3.300 | 3.300 |
Rmerge | 0.061 | 0.653 |
Number of reflections | 56332 | |
<I/σ(I)> | 15.5 | 1.4 |
Completeness [%] | 97.7 | 96.9 |
Redundancy | 5.2 | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.7 | 291 | 7-11% PEG3350, 100 mM MES pH 6.7, 4-15% MPD, VAPOR DIFFUSION, HANGING DROP, temperature 291K |