4OD8

Crystal structure of the vaccinia virus DNA polymerase holoenzyme subunit D4 in complex with the A20 N-terminus

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (B, A)	Uracil-DNA glycosylase	polymer	232	26729.5	2	UniProt (P20536)	Vaccinia virus	UDG
2	B, D (D, C)	DNA polymerase processivity factor component A20	polymer	52	5777.6	2	UniProt (P20995)	Vaccinia virus
3	E, F, G, I, J... (B, A)	SULFATE ION	non-polymer		96.1	6	Chemie (SO4)
4	H, L (B, A)	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
5	M, N, O, P (B, D, A, C)	water	water		18.0	341	Chemie (HOH)

Sequence modifications

B, A: 1 - 218 (UniProt: P20536)

PDB	External Database	Details
Met -13	-	expression tag
Gly -12	-	expression tag
Ser -11	-	expression tag
Ser -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
Ser -3	-	expression tag
Gln -2	-	expression tag
Asp -1	-	expression tag
Pro 0	-	expression tag

D, C: 1 - 50 (UniProt: P20995)

PDB	External Database	Details
Gly -1	-	expression tag
Ala 0	-	expression tag
Ala 2	Thr 2	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	65014.1
Non-Polymers*	Number of molecules	8
	Total formula weight	760.6
All*	Total formula weight	65774.7

*Water molecules are not included.