4OD8
Crystal structure of the vaccinia virus DNA polymerase holoenzyme subunit D4 in complex with the A20 N-terminus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-07-12 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 92.620, 92.620, 145.860 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.310 - 1.850 |
| R-factor | 0.1773 |
| Rwork | 0.175 |
| R-free | 0.21832 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2owq |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.201 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.620 | 1.950 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.072 | 0.542 |
| Number of reflections | 61957 | |
| <I/σ(I)> | 15.8 | 3.5 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 7.1 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.7 | 293 | 100 mM bicine, 1.5 M ammonium sulfate, pH 8.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
