4O22

Binary complex of metal-free PKAc with SP20.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	cAMP-dependent protein kinase catalytic subunit alpha.	polymer	342	40234.8	1	UniProt (P05132) Pfam (PF00069)	Mus musculus (mouse)	PKA C-alpha
2	B (S)	Phosphorylated peptide pSP20.	polymer	20	2199.4	1	UniProt (P61925)	Homo sapiens (human)	PKI-alpha, cAMP-dependent protein kinase inhibitor alpha, cAMP-dependent protein kinase inhibitor, muscle/brain isoform
3	C, D (A, S)	water	water		18.0	271	Chemie (HOH)

Sequence modifications

A: 15 - 350 (UniProt: P05132)

PDB	External Database	Details
His 9	-	expression tag
His 10	-	expression tag
His 11	-	expression tag
His 12	-	expression tag
His 13	-	expression tag
His 14	-	expression tag

S: 605 - 624 (UniProt: P61925)

PDB	External Database	Details
Ala 620	Asn 21	engineered mutation
Ser 621	Ala 22	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	42434.2
All*	Total formula weight	42434.2

*Water molecules are not included.