4NUA
The effects of Lysine 200 and Phenylalanine 239 Farnesyl Pyrophosphate Synthase (FPPS) mutations on the catalytic activity, crystal structure and inhibition by nitrogen containing bisphosphonates
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | Farnesyl pyrophosphate synthase | polymer | 375 | 43070.9 | 1 | UniProt (P14324) Pfam (PF00348) In PDB | Homo sapiens (human) | FPP synthase, FPS, (2E,6E)-farnesyl diphosphate synthase, Dimethylallyltranstransferase, Farnesyl diphosphate synthase, Geranyltranstransferase |
2 | A | 1-HYDROXY-2-(3-PYRIDINYL)ETHYLIDENE BIS-PHOSPHONIC ACID | non-polymer | 283.1 | 1 | Chemie (RIS) | |||
3 | A | MAGNESIUM ION | non-polymer | 24.3 | 3 | Chemie (MG) | |||
4 | A | 1,2-ETHANEDIOL | non-polymer | 62.1 | 6 | Chemie (EDO) | |||
5 | A | PHOSPHATE ION | non-polymer | 95.0 | 1 | Chemie (PO4) | |||
6 | water | water | 18.0 | 385 | Chemie (HOH) |
Sequence modifications
A: 1 - 353 (UniProt: P14324)
PDB | External Database | Details |
---|---|---|
Met -21 | - | expression tag |
Gly -20 | - | expression tag |
Ser -19 | - | expression tag |
Ser -18 | - | expression tag |
His -17 | - | expression tag |
His -16 | - | expression tag |
His -15 | - | expression tag |
His -14 | - | expression tag |
His -13 | - | expression tag |
His -12 | - | expression tag |
Ser -11 | - | expression tag |
Ser -10 | - | expression tag |
Gly -9 | - | expression tag |
Arg -8 | - | expression tag |
Glu -7 | - | expression tag |
Asn -6 | - | expression tag |
Leu -5 | - | expression tag |
Tyr -4 | - | expression tag |
Phe -3 | - | expression tag |
Gln -2 | - | expression tag |
Gly -1 | - | expression tag |
His 0 | - | expression tag |
Ala 9 | Val 75 | conflict |
Thr 53 | Ala 119 | conflict |
Ala 239 | Phe 305 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 1 |
Total formula weight | 43070.9 | |
Non-Polymers* | Number of molecules | 11 |
Total formula weight | 823.4 | |
All* | Total formula weight | 43894.3 |