4NRK

Structure of hemagglutinin with F95Y mutation of influenza virus B/Lee/40 complex with LSTc

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C, E (A, C, E)	Hemagglutinin HA1 chain	polymer	346	37756.1	3	UniProt (P03460) Pfam (PF00509)	Influenza B virus
2	B, D, F (B, D, F)	Hemagglutinin HA2 chain	polymer	182	19543.0	3	UniProt (P03460) Pfam (PF00509)	Influenza B virus
3	G, H, I, J, L... (G, H, I, J, L...)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	12	In PDB GlyTouCan (G42666HT)
4	K, P, V (K, P, V)	N-acetyl-alpha-neuraminic acid-(2-6)-beta-D-galactopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		674.6	3	In PDB BIRD (PRD_900046) GlyTouCan (G73578JC)		6'-sialyl-N-acetyllactosamine
5	M (M)	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		586.5	1	In PDB GlyTouCan (G15407YE)
6	AA, BA, W, X, Y... (E, F, A, B, C...)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	6	Chemie (NAG)
7	CA, DA, EA, FA, GA... (A, B, C, D, E...)	water	water		18.0	350	Chemie (HOH)

Sequence modifications

A, C, E: 1 - 346 (UniProt: P03460)

PDB	External Database	Details
Arg 38	Lys 53	conflict
Ile 76	Thr 91	conflict
Val 90	Ala 105	conflict
Tyr 95	Phe 110	engineered mutation
Thr 147	Ala 162	conflict
Ile 167	Thr 182	conflict

B, D, F: 1 - 176 (UniProt: P03460)

PDB	External Database	Details
Ser 54	Tyr 415	conflict
Gly 177	-	expression tag
Ala 178	-	expression tag
Leu 179	-	expression tag
Val 180	-	expression tag
Pro 181	-	expression tag
Arg 182	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	171897.2
Branched	Number of molecules	16
	Total formula weight	7703.2
Non-Polymers*	Number of molecules	6
	Total formula weight	1327.2
All*	Total formula weight	180927.6

*Water molecules are not included.