4NRK
Structure of hemagglutinin with F95Y mutation of influenza virus B/Lee/40 complex with LSTc
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 200 |
Detector technology | CCD |
Collection date | 2013-08-09 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97872A |
Spacegroup name | P 21 2 21 |
Unit cell lengths | 83.610, 128.300, 211.810 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.873 - 2.630 |
R-factor | 0.2085 |
Rwork | 0.207 |
R-free | 0.24480 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.003 |
RMSD bond angle | 0.839 |
Refinement software | PHENIX ((phenix.refine: dev_1452)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.460 | 2.770 |
High resolution limit [Å] | 2.630 | 2.630 |
Rmerge | 0.111 | 0.499 |
Number of reflections | 68564 | |
<I/σ(I)> | 11.5 | 2.4 |
Completeness [%] | 100.0 | 100 |
Redundancy | 5.3 | 5.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 290 | 0.1 M di-Sodium succinate 15% w/v PEG3350, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 290K |