4NQL
The crystal structure of the DUB domain of AMSH orthologue, Sst2 from S. pombe, in complex with lysine 63-linked diubiquitin
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | AMSH-like protease sst2 | polymer | 221 | 24661.0 | 1 | UniProt (Q9P371) Pfam (PF01398) | Schizosaccharomyces pombe (Fission yeast) | Suppressor of ste12 deletion protein 2 |
| 2 | B (B) | Ubiquitin | polymer | 76 | 8604.8 | 1 | UniProt (P0CG50) Pfam (PF00240) | Mus musculus (mouse) | Ubiquitin, Ubiquitin-related 1, Ubiquitin-related 2 |
| 3 | C (C) | Ubiquitin | polymer | 77 | 8691.9 | 1 | UniProt (P0CG50) Pfam (PF00240) | Mus musculus (mouse) | Ubiquitin, Ubiquitin-related 1, Ubiquitin-related 2 |
| 4 | D (A) | ZINC ION | non-polymer | 65.4 | 1 | Chemie (ZN) | |||
| 5 | E, F, G, H, I... (A, B, C) | 1,2-ETHANEDIOL | non-polymer | 62.1 | 7 | Chemie (EDO) | |||
| 6 | L, M, N (A, B, C) | water | water | 18.0 | 48 | Chemie (HOH) |
Sequence modifications
A: 221 - 435 (UniProt: Q9P371)
B: 1 - 76 (UniProt: P0CG50)
C: 1 - 76 (UniProt: P0CG50)
| PDB | External Database | Details |
|---|---|---|
| Gly 215 | - | expression tag |
| Pro 216 | - | expression tag |
| Leu 217 | - | expression tag |
| Gly 218 | - | expression tag |
| Ser 219 | - | expression tag |
| Met 220 | - | expression tag |
| Ala 234 | Tyr 234 | conflict |
| Val 235 | Thr 235 | conflict |
| Lys 239 | Glu 239 | conflict |
| Ala 286 | Glu 286 | conflict |
| Ala 354 | Asp 354 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Arg 63 | Lys 63 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Asp 77 | - | insertion |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 41957.7 | |
| Non-Polymers* | Number of molecules | 8 |
| Total formula weight | 499.9 | |
| All* | Total formula weight | 42457.6 |
