4NQL

The crystal structure of the DUB domain of AMSH orthologue, Sst2 from S. pombe, in complex with lysine 63-linked diubiquitin

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	AMSH-like protease sst2	polymer	221	24661.0	1	UniProt (Q9P371) Pfam (PF01398) In PDB	Schizosaccharomyces pombe (Fission yeast)	Suppressor of ste12 deletion protein 2
2	B	Ubiquitin	polymer	76	8604.8	1	UniProt (P0CG50) Pfam (PF00240) In PDB	Mus musculus (mouse)	Ubiquitin, Ubiquitin-related 1, Ubiquitin-related 2
3	C	Ubiquitin	polymer	77	8691.9	1	UniProt (P0CG50) Pfam (PF00240) In PDB	Mus musculus (mouse)	Ubiquitin, Ubiquitin-related 1, Ubiquitin-related 2
4	A	ZINC ION	non-polymer		65.4	1	Chemie (ZN)
5	A, B, C	1,2-ETHANEDIOL	non-polymer		62.1	7	Chemie (EDO)
6		water	water		18.0	48	Chemie (HOH)

Sequence modifications

A: 221 - 435 (UniProt: Q9P371)

PDB	External Database	Details
Gly 215	-	expression tag
Pro 216	-	expression tag
Leu 217	-	expression tag
Gly 218	-	expression tag
Ser 219	-	expression tag
Met 220	-	expression tag
Ala 234	Tyr 234	conflict
Val 235	Thr 235	conflict
Lys 239	Glu 239	conflict
Ala 286	Glu 286	conflict
Ala 354	Asp 354	conflict

B: 1 - 76 (UniProt: P0CG50)

PDB	External Database	Details
Arg 63	Lys 63	engineered mutation

C: 1 - 76 (UniProt: P0CG50)

PDB	External Database	Details
Asp 77	-	insertion

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	41957.7
Non-Polymers*	Number of molecules	8
	Total formula weight	499.9
All*	Total formula weight	42457.6

*Water molecules are not included.