4NM7

Crystal structure of GSK-3/Axin complex bound to phosphorylated Wnt receptor LRP6 e-motif

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	GSK3B protein	polymer	377	42857.2	1	UniProt (Q6FI27) Pfam (PF00069) UniProt (by SIFTS) (P49841)	Homo sapiens (human)	GSK3beta isoform, Glycogen synthase kinase 3 beta, isoform CRA_b, cDNA FLJ75266, highly similar to Homo sapiens glycogen synthase kinase 3 beta, mRNA
2	B (B)	Axin-1	polymer	24	2738.1	1	UniProt (O15169)	Homo sapiens (human)	Axis inhibition protein 1, hAxin
3	C (C)	Phosphorylated Wnt receptor LRP6 e-motif	polymer	9	1006.1	1	UniProt (by SIFTS) (O75581)	Homo sapiens
4	D (A)	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	1	Chemie (ADP)
5	E, F, G (A)	GLYCEROL	non-polymer		92.1	3	Chemie (GOL)
6	H, I (A)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
7	J (A)	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
8	K (A)	2,3-DIHYDROXY-1,4-DITHIOBUTANE	non-polymer		154.3	1	Chemie (DTT)
9	L, M, N (A, B, C)	water	water		18.0	106	Chemie (HOH)

Sequence modifications

A: 13 - 383 (UniProt: Q6FI27)

PDB	External Database	Details
His 384	-	expression tag
His 385	-	expression tag
His 386	-	expression tag
His 387	-	expression tag
His 388	-	expression tag
His 389	-	expression tag

B: 383 - 402 (UniProt: O15169)

PDB	External Database	Details
Gly 379	-	expression tag
Gly 380	-	expression tag
Ile 381	-	expression tag
Leu 382	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	46601.4
Non-Polymers*	Number of molecules	8
	Total formula weight	941.8
All*	Total formula weight	47543.2

*Water molecules are not included.