4NI2

Crystal structure of the heterodimeric catalytic domain of wild-type human soluble guanylate cyclase

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Guanylate cyclase soluble subunit alpha-3	polymer	197	21786.3	1	UniProt (Q02108) Pfam (PF00211) In PDB	Homo sapiens (human)	GCS-alpha-3, GCS-alpha-1, Soluble guanylate cyclase large subunit
2	B	Guanylate cyclase soluble subunit beta-1	polymer	209	23567.1	1	UniProt (Q02153) Pfam (PF00211) In PDB	Homo sapiens (human)	GCS-beta-1, Guanylate cyclase soluble subunit beta-3, GCS-beta-3, Soluble guanylate cyclase small subunit
3	A, B	1,2-ETHANEDIOL	non-polymer		62.1	6	Chemie (EDO)
4		water	water		18.0	310	Chemie (HOH)

Sequence modifications

A: 468 - 662 (UniProt: Q02108)

PDB	External Database	Details
Ser 466	-	expression tag
Met 467	-	expression tag

B: 408 - 608 (UniProt: Q02153)

PDB	External Database	Details
Met 407	-	expression tag
Ala 609	-	expression tag
Glu 610	-	expression tag
Asn 611	-	expression tag
Leu 612	-	expression tag
Tyr 613	-	expression tag
Phe 614	-	expression tag
Gln 615	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	45353.3
Non-Polymers*	Number of molecules	6
	Total formula weight	372.4
All*	Total formula weight	45725.7

*Water molecules are not included.