4NDI

Human Aprataxin (Aptx) AOA1 variant K197Q bound to RNA-DNA, AMP, and Zn - product complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Aprataxin	polymer	182	21258.7	2	UniProt (Q7Z2E3) Pfam (PF11969) Pfam (PF16278) In PDB	Homo sapiens (human)	Forkhead-associated domain histidine triad-like protein, FHA-HIT
2	D, G	5'-R(P*G)-D(P*TP*TP*AP*TP*GP*AP*TP*TP*C)-3'	polymer	10	3066.0	2
3	E, H	5'-D(*GP*AP*AP*TP*CP*AP*TP*AP*AP*C)-3'	polymer	10	3037.0	2
4	A, B	ADENOSINE MONOPHOSPHATE	non-polymer		347.2	2	Chemie (AMP)
5	A, B	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
6	A	SODIUM ION	non-polymer		23.0	1	Chemie (NA)
7	A, B	BETA-MERCAPTOETHANOL	non-polymer		78.1	2	Chemie (BME)
8	A	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
9	D, G	POTASSIUM ION	non-polymer		39.1	2	Chemie (K)
10		water	water		18.0	698	Chemie (HOH)

Sequence modifications

A, B: 165 - 342 (UniProt: Q7Z2E3)

PDB	External Database	Details
Gly 161	-	expression tag
Ser 162	-	expression tag
His 163	-	expression tag
Met 164	-	expression tag
Gln 197	Lys 211	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	54723.5
Non-Polymers*	Number of molecules	10
	Total formula weight	1174.8
All*	Total formula weight	55898.3

*Water molecules are not included.