4NDI

Human Aprataxin (Aptx) AOA1 variant K197Q bound to RNA-DNA, AMP, and Zn - product complex

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003824	molecular_function	catalytic activity
B	0003824	molecular_function	catalytic activity

Functional Information from PDB Data

site_id	AC1
Number of Residues	20
Details	BINDING SITE FOR RESIDUE AMP A 401

Chain	Residue
A	GLY170
A	SER255
A	MET256
A	HIS260
A	HIS262
A	HOH573
A	HOH577
A	HOH661
A	HOH665
A	HOH710
D	G1
A	LEU171
D	HOH229
A	SER174
A	MET180
A	ASP193
A	LYS194
A	TYR195
A	HIS251
A	PRO254

---

site_id	AC2
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN A 402

Chain	Residue
A	CYS319
A	CYS322
A	HIS335
A	HIS339

---

site_id	AC3
Number of Residues	6
Details	BINDING SITE FOR RESIDUE NA A 403

Chain	Residue
A	SER255
A	HOH554
A	HOH625
D	HOH211
D	HOH218
D	HOH242

---

site_id	AC4
Number of Residues	6
Details	BINDING SITE FOR RESIDUE BME A 404

Chain	Residue
A	TYR195
A	CYS272
A	LYS274
A	HOH519
B	GLN298
D	DT3

---

site_id	AC5
Number of Residues	3
Details	BINDING SITE FOR RESIDUE GOL A 405

Chain	Residue
A	SER211
A	SER257
A	HOH532

---

site_id	AC6
Number of Residues	21
Details	BINDING SITE FOR RESIDUE AMP B 401

Chain	Residue
B	LEU171
B	SER174
B	ILE191
B	LYS192
B	ASP193
B	LYS194
B	TYR195
B	LEU203
B	HIS251
B	SER255
B	MET256
B	HIS260
B	HIS262
B	HOH507
B	HOH524
B	HOH531
B	HOH559
B	HOH616
B	HOH617
B	HOH618
G	G1

---

site_id	AC7
Number of Residues	4
Details	BINDING SITE FOR RESIDUE ZN B 402

Chain	Residue
B	CYS319
B	CYS322
B	HIS335
B	HIS339

---

site_id	AC8
Number of Residues	5
Details	BINDING SITE FOR RESIDUE BME B 403

Chain	Residue
B	TYR195
B	CYS272
B	LYS274
G	DT3
G	HOH203

---

site_id	AC9
Number of Residues	7
Details	BINDING SITE FOR RESIDUE K D 101

Chain	Residue
D	DT8
D	DT9
D	HOH220
D	HOH240
E	DT4
E	DC5
E	HOH142

---

site_id	BC1
Number of Residues	6
Details	BINDING SITE FOR RESIDUE K G 101

Chain	Residue
G	DT8
G	DT9
G	HOH213
G	HOH218
H	DT4
H	DC5

---

Functional Information from PROSITE/UniProt

site_id	PS00028
Number of Residues	21
Details	ZINC_FINGER_C2H2_1 Zinc finger C2H2 type domain signature. Che..CqqlLpsipqlkeHlrk..H

Chain	Residue	Details
A	CYS319-HIS339

---

site_id	PS00892
Number of Residues	19
Details	HIT_1 HIT domain signature. RlgyhAiPsMsHVHLHVIS

Chain	Residue	Details
A	ARG247-SER265

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	210
Details	Domain: {"description":"HIT","evidences":[{"source":"PROSITE-ProRule","id":"PRU00464","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	44
Details	Zinc finger: {"description":"C2H2-type","evidences":[{"source":"PROSITE-ProRule","id":"PRU00042","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"17276982","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"24362567","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	10
Details	Region: {"description":"Interaction with DNA substrate","evidences":[{"source":"PubMed","id":"24362567","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	8
Details	Motif: {"description":"Histidine triad motif","evidences":[{"source":"PROSITE-ProRule","id":"PRU00464","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"24362567","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	2
Details	Active site: {"description":"Tele-AMP-histidine intermediate","evidences":[{"source":"PubMed","id":"24362567","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	8
Details	Site: {"description":"Interaction with DNA substrate","evidences":[{"source":"PubMed","id":"24362567","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---