4N5Y

Crystal structure of H5 hemagglutinin mutant (N158D, N224K and Q226L) from the influenza virus A/Viet Nam/1203/2004 (H5N1)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C, E, G, I...	Hemagglutinin HA1 chain	polymer	334	37949.1	15	UniProt (Q6DQ33) Pfam (PF00509) In PDB	Influenza A virus
2	B, D, F, H, J...	Hemagglutinin HA2 chain	polymer	181	20881.1	15	UniProt (Q6DQ33) Pfam (PF00509) In PDB	Influenza A virus
3	e, g, h, j, l...	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		586.5	10	In PDB GlyTouCan (G15407YE)
4	0, 1, f, i, k...	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	13	In PDB GlyTouCan (G42666HT)
5	s	alpha-D-mannopyranose-(1-3)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		748.7	1	In PDB GlyTouCan (G81315DD)
6	K, O, Y, c	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	5	Chemie (NAG)

Sequence modifications

A, C, E, G, I, K, M, O, Q, S, U, W, Y, a, c: 11 - 333 (UniProt: Q6DQ33)

PDB	External Database	Details
Ala 7	-	expression tag
Asp 8	-	expression tag
Pro 9	-	expression tag
Gly 10	-	expression tag
Asp 158	Asn 170	engineered mutation
Lys 224	Asn 236	engineered mutation
Leu 226	Gln 238	engineered mutation

B, D, F, H, J, L, N, P, R, T, V, X, Z, b, d: 1 - 174 (UniProt: Q6DQ33)

PDB	External Database	Details
Ser 175	-	expression tag
Gly 176	-	expression tag
Arg 177	-	expression tag
Leu 178	-	expression tag
Val 179	-	expression tag
Pro 180	-	expression tag
Arg 181	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	30
	Total formula weight	882453.3
Branched	Number of molecules	24
	Total formula weight	12131.3
Non-Polymers*	Number of molecules	5
	Total formula weight	1106.0
All*	Total formula weight	895690.6

*Water molecules are not included.