4N5Y

Crystal structure of H5 hemagglutinin mutant (N158D, N224K and Q226L) from the influenza virus A/Viet Nam/1203/2004 (H5N1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	CCD
Collection date	2012-06-27
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.05876
Spacegroup name	P 1
Unit cell lengths	117.915, 118.105, 273.799
Unit cell angles	91.50, 90.18, 119.87

Refinement procedure

Resolution	45.283 - 3.162
R-factor	0.215
Rwork	0.213
R-free	0.26150
Structure solution method	Phaser
Starting model (for MR)	3gbm
RMSD bond length	0.012
RMSD bond angle	1.555
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.270
High resolution limit [Å]	3.162	3.162
Number of reflections	199766
<I/σ(I)>	7.1
Completeness [%]	93.6	83.4
Redundancy	1.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	295	0.1 M HEPES, pH 7.5, 0.2 KF, 22% (w/v) PEG3350, VAPOR DIFFUSION, SITTING DROP, temperature 295K