4N4I

Crystal structure of the Bromo-PWWP of the mouse zinc finger MYND-type containing 11 isoform alpha in complex with histone H3.3K36me3

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Zinc finger MYND domain-containing protein 11	polymer	252	29503.7	1	UniProt (Q8R5C8) Pfam (PF00439) Pfam (PF00855)	Mus musculus (mouse)	Adenovirus 5 E1A-binding protein, Protein BS69
2	B (B)	Peptide from Histone H3.3	polymer	24	2659.1	1	UniProt (P84243)	Homo sapiens
3	C (A)	ZINC ION	non-polymer		65.4	1	Chemie (ZN)
4	D (A)	PHOSPHATE ION	non-polymer		95.0	1	Chemie (PO4)
5	E (A)	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	1	Chemie (PEG)
6	F, G (A, B)	water	water		18.0	116	Chemie (HOH)

Sequence modifications

A: 154 - 371 (UniProt: Q8R5C8)

PDB	External Database	Details
Met 120	-	expression tag
Gly 121	-	expression tag
Ser 122	-	expression tag
Ser 123	-	expression tag
His 124	-	expression tag
His 125	-	expression tag
His 126	-	expression tag
His 127	-	expression tag
His 128	-	expression tag
His 129	-	expression tag
Ser 130	-	expression tag
Ser 131	-	expression tag
Gly 132	-	expression tag
Leu 133	-	expression tag
Val 134	-	expression tag
Pro 135	-	expression tag
Arg 136	-	expression tag
Gly 137	-	expression tag
Ser 138	-	expression tag
His 139	-	expression tag
Met 140	-	expression tag
Ala 141	-	expression tag
Ser 142	-	expression tag
Met 143	-	expression tag
Thr 144	-	expression tag
Gly 145	-	expression tag
Gly 146	-	expression tag
Gln 147	-	expression tag
Gln 148	-	expression tag
Met 149	-	expression tag
Gly 150	-	expression tag
Arg 151	-	expression tag
Gly 152	-	expression tag
Ser 153	-	expression tag
Ala 234	Asp 234	engineered mutation
Ala 236	Glu 236	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	32162.9
Non-Polymers*	Number of molecules	3
	Total formula weight	266.5
All*	Total formula weight	32429.4

*Water molecules are not included.