4N0A

Crystal structure of Lsm2-3-Pat1C complex from Saccharomyces cerevisiae

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, E, F (A, B, E, F)	U6 snRNA-associated Sm-like protein LSm3	polymer	89	10039.3	4	UniProt (P57743) Pfam (PF01423)	Saccharomyces cerevisiae (Baker's yeast)	SmX4 protein
2	C, D, G (C, D, G)	U6 snRNA-associated Sm-like protein LSm2	polymer	109	12796.6	3	UniProt (P38203) Pfam (PF01423)	Saccharomyces cerevisiae (Baker's yeast)	Small nuclear ribonucleoprotein D homolog SNP3
3	H, I, J (H, I, J)	DNA topoisomerase 2-associated protein PAT1	polymer	380	43190.5	3	UniProt (P25644) Pfam (PF09770)	Saccharomyces cerevisiae (Baker's yeast)	Decapping activator and translational repressor PAT1, Topoisomerase II-associated protein PAT1, mRNA turnover protein 1
4	K, L, M, N, O... (A, B, C, D, E...)	water	water		18.0	77	Chemie (HOH)

Sequence modifications

C, D, G: 1 - 95 (UniProt: P38203)

PDB	External Database	Details
Met -13	-	expression tag
Gly -12	-	expression tag
Ser -11	-	expression tag
Ser -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
Ser -3	-	expression tag
Gln -2	-	expression tag
Asp -1	-	expression tag
Pro 0	-	expression tag

H, I, J: 422 - 796 (UniProt: P25644)

PDB	External Database	Details
Gly 417	-	expression tag
Pro 418	-	expression tag
Leu 419	-	expression tag
Gly 420	-	expression tag
Ser 421	-	expression tag
Val 688	Asp 688	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	10
	Total formula weight	208118.2
All*	Total formula weight	208118.2

*Water molecules are not included.