4N0A
Crystal structure of Lsm2-3-Pat1C complex from Saccharomyces cerevisiae
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-11-03 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.072, 1.2553, 1.2557, 1.2427 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 138.447, 251.511, 120.310 |
| Unit cell angles | 90.00, 112.09, 90.00 |
Refinement procedure
| Resolution | 19.884 - 3.150 |
| R-factor | 0.2736 |
| Rwork | 0.272 |
| R-free | 0.30020 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.113 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.6.1_357)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.900 | 3.262 |
| High resolution limit [Å] | 3.150 | 3.150 |
| Number of reflections | 65011 | |
| Completeness [%] | 99.6 | 99.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 288 | 0.1M Tris, 12% ethanol, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 288K |
