4N07

Crystal structure of the GluA2 ligand-binding domain (S1S2J-L483Y-N754S) in complex with glutamate and BPAM-344 at 1.87 A resolution

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C	Glutamate receptor 2	polymer	263	29244.7	3	UniProt (P19491) Pfam (PF10613) Pfam (PF00060) Pfam (PF00497) In PDB	Rattus norvegicus (rat)	GluR-2, AMPA-selective glutamate receptor 2, GluR-B, GluR-K2, Glutamate receptor ionotropic, AMPA 2, GluA2
2	A, C, B	4-cyclopropyl-7-fluoro-3,4-dihydro-2H-1,2,4-benzothiadiazine 1,1-dioxide	non-polymer		242.3	3	Chemie (2J9)
3	A, C, B	GLUTAMIC ACID	non-polymer		147.1	3	Chemie (GLU)
4	A, C, B	ACETATE ION	non-polymer		59.0	13	Chemie (ACT)
5	B, C, A	ZINC ION	non-polymer		65.4	10	Chemie (ZN)
6	A	CACODYLATE ION	non-polymer		137.0	1	Chemie (CAC)
7	B, A	GLYCEROL	non-polymer		92.1	6	Chemie (GOL)
8		water	water		18.0	687	Chemie (HOH)

Sequence modifications

A, B, C: 3 - 117 (UniProt: P19491)

PDB	External Database	Details
Gly 1	-	expression tag
Ala 2	-	expression tag
Tyr 94	Leu 504	engineered mutation

A, B, C: 120 - 263 (UniProt: P19491)

PDB	External Database	Details
Gly 118	-	linker
Thr 119	-	linker
Ser 242	Asn 775	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	87734.0
Non-Polymers*	Number of molecules	36
	Total formula weight	3279.4
All*	Total formula weight	91013.4

*Water molecules are not included.