4MWT

Crystal structure of human PPCA (trigonal crystal form 2)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Lysosomal protective protein	polymer	428	48655.6	2	UniProt (P10619) Pfam (PF00450)	Homo sapiens (human)	Carboxypeptidase C, Carboxypeptidase L, Cathepsin A, Protective protein cathepsin A, PPCA, Protective protein for beta-galactosidase
2	C (C)	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		586.5	1	In PDB GlyTouCan (G15407YE)
3	D, E, F (A, B)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	3	Chemie (NAG)

Sequence modifications

A, B: 1 - 452 (UniProt: P10619)

PDB	External Database	Details
-	Tyr 291	deletion
-	Glu 292	deletion
-	Lys 293	deletion
-	Asp 294	deletion
-	Thr 295	deletion
-	Val 296	deletion
-	Val 297	deletion
-	Val 298	deletion
-	Gln 299	deletion
-	Asp 300	deletion
-	Leu 301	deletion
-	Gly 302	deletion
-	Asn 303	deletion
-	Ile 304	deletion
-	Phe 305	deletion
-	Thr 306	deletion
-	Arg 307	deletion
-	Leu 308	deletion
-	Pro 309	deletion
-	Leu 310	deletion
-	Lys 311	deletion
-	Arg 312	deletion
-	Met 313	deletion
-	Trp 314	deletion
-	His 315	deletion
-	Gln 316	deletion
-	Ala 317	deletion
-	Leu 318	deletion
-	Leu 319	deletion
-	Arg 320	deletion
His 453	-	expression tag
His 454	-	expression tag
His 455	-	expression tag
His 456	-	expression tag
His 457	-	expression tag
His 458	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	97311.2
Branched	Number of molecules	1
	Total formula weight	586.5
Non-Polymers*	Number of molecules	3
	Total formula weight	663.6
All*	Total formula weight	98561.3

*Water molecules are not included.