4MQT

Structure of active human M2 muscarinic acetylcholine receptor bound to the agonist iperoxo and allosteric modulator LY2119620

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Muscarinic acetylcholine receptor M2	polymer	351	39951.7	1	UniProt (P08172) Pfam (PF00001) In PDB	Homo sapiens (human)
2	B	Nanobody 9-8	polymer	125	13428.4	1		Lama glama
3	A	4-(4,5-dihydro-1,2-oxazol-3-yloxy)-N,N,N-trimethylbut-2-yn-1-aminium	non-polymer		197.3	1	Chemie (IXO)
4	A	3-amino-5-chloro-N-cyclopropyl-4-methyl-6-[2-(4-methylpiperazin-1-yl)-2-oxoethoxy]thieno[2,3-b]pyridine-2-carboxamide	non-polymer		437.9	1	Chemie (2CU)

Sequence modifications

A: -1 - 232 (UniProt: P08172)

PDB	External Database	Details
Asp -16	-	expression tag
Tyr -15	-	expression tag
Lys -14	-	expression tag
Asp -13	-	expression tag
Asp -12	-	expression tag
Asp -11	-	expression tag
Glu -10	-	expression tag
Asn -9	-	expression tag
Leu -8	-	expression tag
Tyr -7	-	expression tag
Phe -6	-	expression tag
Gln -5	-	expression tag
Gly -4	-	expression tag
Leu -3	-	expression tag
Glu -2	-	expression tag
Asp 0	Asn 2	engineered mutation
Asp 1	Asn 3	engineered mutation
Asp 4	Asn 6	engineered mutation
Asp 7	Asn 9	engineered mutation
Leu 17	-	insertion
Glu 18	-	insertion
His 467	-	expression tag
His 468	-	expression tag
His 469	-	expression tag
His 470	-	expression tag
His 471	-	expression tag
His 472	-	expression tag
His 473	-	expression tag
His 474	-	expression tag

A: 373 - 466 (UniProt: P08172)

PDB	External Database	Details
Thr 373	Ala 373	engineered mutation
Arg 374	Lys 374	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	53380.1
Non-Polymers*	Number of molecules	2
	Total formula weight	635.2
All*	Total formula weight	54015.3

*Water molecules are not included.