4MP0

Structure of a second nuclear PP1 Holoenzyme, crystal form 2

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, C)	Serine/threonine-protein phosphatase PP1-alpha catalytic subunit	polymer	299	34162.1	2	UniProt (P62136) Pfam (PF16891) Pfam (PF00149)	Homo sapiens (human)	PP-1A
2	C, D (B, D)	Serine/threonine-protein phosphatase 1 regulatory subunit 10	polymer	44	5218.9	2	UniProt (O55000)	Rattus norvegicus (brown rat rat rats)	MHC class I region proline-rich protein CAT53, Phosphatase 1 nuclear targeting subunit, Protein PNUTS
3	E, F, J, K (A, C)	MANGANESE (II) ION	non-polymer		54.9	4	Chemie (MN)
4	G, H (A)	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
5	I, L (A, C)	PHOSPHATE ION	non-polymer		95.0	2	Chemie (PO4)
6	M, N, O, P (A, C, B, D)	water	water		18.0	315	Chemie (HOH)

Sequence modifications

A, C: 7 - 300 (UniProt: P62136)

PDB	External Database	Details
Gly 2	-	expression tag
His 3	-	expression tag
Met 4	-	expression tag
Gly 5	-	expression tag
Ser 6	-	expression tag

B, D: 394 - 433 (UniProt: O55000)

PDB	External Database	Details
Gly 390	-	expression tag
Ala 391	-	expression tag
Met 392	-	expression tag
Gly 393	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	78762.0
Non-Polymers*	Number of molecules	8
	Total formula weight	593.9
All*	Total formula weight	79355.9

*Water molecules are not included.